||15:00 - 16:15
|Sophisticated diagnostic and therapeutic approaches for ocular surface diseases Joint Symposium of the Hungarian Ophthalmological Society and the DOG
Vorsitzende/r: Zoltán Zsolt Nagy (Budapest), Ildikó Süveges (Budapest), Berthold Seitz (Homburg/Saar)
This traditional symposium of the Hungarian Ophthalmological Society and the DOG will include up-to-date diagnostic and therapeutic approaches for commonly diagnosed corneal diseases. Clinical, biochemical, tomographic and confocal microscopic aspects of corneal wound healing will be covered. New insights in the content of autologous serum and amniotic membrane suspension may affect corneal epithelial cell and keratocyte viability, proliferation and migration. In addition, the potential favorable outcome of femtosecond laser assisted PKP for elective indications and emergency PKP for acute acanthamoeba keratitis will be discussed.
Referent/in: Ildikó Süveges
Wound healing in the cornea is different from that of other tissues for these three reasons: 1) the cornea does not have blood vessels/has no blood supply/ it is avascular 2) it has immune privilege/ it is an immune privileged tissue/immunologic privilege makes it a very special tissue 3) it has a very slow metabolism . Corneal wound healing can be examined when the injury is vertical to the surface: horizontal cuts will not elicit reactions provoked by vertical incisions (see lamellar keratoplasty). Wound healing in the various layers of the cornea induces various processes the most important of which being epithelial injuries/abrasions that release cytokines. They, in turn, trigger such processes as the degradation of extracellular matrix, the apoptosis of keratocytes or the chemotaxis of leucocytes. The restoration of the epidermis is performed through the process of re-epithelialization in which keratocytes play an active role. The characteristics of the newly generated scar tissue are different from that of the normal cornea: it is not transparent, its strength being 50% less. In cases of pathologic wound healing retrocorneal membrane and pannus-like tissue may develop. Corneal scarring can impair vision. It can only be managed by surgery.
Referent/in: Zoltán Zsolt Nagy
Authors have examined the role of posterior elevation in keratoconus and its change following cross linking (CXL) therapy of the cornea. The epi-off technique resulted in improvement in posterior elevation, which explains the favorable visual changes using CXL technology in keratoconus patients.
Referent/in: Miklós Resch
Confocal corneal microscopy provides insight into all cellular and extracellular components playing role in the wound healing of the cornea. We give a review of the wound healing process after penetrating keratoplasty and other corneal surgeries such as refractive surgery, with a special focus on Langerhans cells and nerve regeneration.The effect of autoimmune disease and dry eye syndrome, furthermore topical use of different medications will be also discussed.
Referent/in: Nora Szentmáry
We analysed the effect of AS and AMS on human epithelial cells and keratocytes. For the best viability and migration of HCEC 30% AS, for proliferation 15% AS should be used. With unchanged HCEC viability and proliferation and increased HCEC migration, 15% and 30% AMS application seems to be the most appropriate method to support epithelial healing. The interindividual variability of growth factor concentration in AS and AMS should be taken into account before clinical use.
Referent/in: László Módis
Femtosecond laser-assisted keratoplasty (FLAK) assures automatic and safe trephination of donor and recipient corneas. Our results indicate that precise graft-host alignment provides stable anatomical and functional corneal graft parameters in the early postoperative period and fast patient rehabilitation can be achieved. However, FLAK has its limitation (e.g. trephination of thick or vascularised corneas) moreover, at the moment results are not superior in comparison with conventional keratoplasty techniques.
Referent/in: Kornélia Lenke Laurik
Still, acanthamoeba keratitis (AK) represents a diagnostic and therapeutic challenge. Vast majority of experts recommend corneal transplantation months after the active phase of the AK. According to our results, penetrating keratoplasty à chaud in the active phase of therapy-resistant AK could be a reasonable alternative for a faster resolution.